Faili:Summary of SAGE.svg

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Maelezo
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1]
Tarehe
Chanzo Kazi yangu
Mwandishi Thomas Shafee
Matoleo mengine

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

Hatimiliki

I, the copyright holder of this work, hereby publish it under the following license:
w:en:Creative Commons
sifa
This file is licensed under the Creative Commons Attribution 4.0 International license.
Uko huru:
  • kushiriki – kunakili na kusambaza kazi hiyo
  • kwa remix – kurekebisha kazi
Chini ya masharti yafuatayo:
  • sifa – Ni lazima utoe mkopo unaofaa, utoe kiungo cha leseni, na uonyeshe ikiwa mabadiliko yalifanywa. Unaweza kufanya hivyo kwa njia yoyote inayofaa, lakini si kwa njia yoyote inayopendekeza mtoa leseni akuidhinishe wewe au matumizi yako.
  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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Serial analysis of gene expression Kiingereza

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kuanzishwa

5 Oktoba 2016

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Tarehe/SaaPicha ndogoVipimoMtumiajiMaelezo
sasa hivi12:21, 13 Februari 2017Picha ndogo ya toleo la 12:21, 13 Februari 2017938 × 756 (180 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 Februari 2017Picha ndogo ya toleo la 10:48, 13 Februari 2017938 × 756 (104 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
05:27, 7 Oktoba 2016Picha ndogo ya toleo la 05:27, 7 Oktoba 2016938 × 756 (104 KB)Evolution and evolvabilityce
05:23, 7 Oktoba 2016Picha ndogo ya toleo la 05:23, 7 Oktoba 2016938 × 756 (102 KB)Evolution and evolvabilityce
05:16, 7 Oktoba 2016Picha ndogo ya toleo la 05:16, 7 Oktoba 2016938 × 756 (104 KB)Evolution and evolvabilitydigitag-->tag
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